The ad compares buying a car to finding a wife, saying "an important decision must be made carefully".
It shows a woman having her nose, ears and teeth inspected by her mother-in-law on her wedding day.
An Audi spokesman told the South China Morning Post marketing in China was the responsibility of its local joint venture partner.
Some are calling for a boycott of the German carmaker - which is recognised as one of the big three in China, along with Mercedes Benz and BMW.
One user called it "a terrible ad!" with others branding it "disgusting".