The investigation unit known as Strike Force Nuralda has asked betting agencies to reveal every gambling transaction on every market for Sea Eagles games in 2015 and 2016, according to News Corp Australia report.
The news comes as Sea Eagles chairman Scott Penn claimed Manly was a victim of "guilt by innuendo", revealing the club had yet to be contacted by police.
"We are concerned about the way in which the club brand has been tarnished by speculation," Penn said.
"It is guilt by innuendo."
"We have reached out to (the police) and literally had one conversation and they said we will get back to you when we are ready."
It's believed police had only previously requested betting information for two matches last year, before the strike force was launched earlier this week.
Meanwhile, Fairfax Media is reporting Ladbrokes rejected a $30,000 deposit into former Kiwis, Manly and Parramatta playmaker Kieran Foran's betting account this year.
It's reported that the organisation had grown concerned about the negative publicity regarding Foran's gambling habits, and believed the deposit was out of character for the Eels' captain's account.