A joint streaming release, it sets a pandemic-era record for a family film.
The live-action/animated basketball film - a sequel to the 1996 original starring Michael Jordan - sees NBA star LeBron James team up with the Looney Tunes characters to save his son.
Its success, despite poor reviews, means it topples Marvel's Black Widow.
The superhero adventure, starring Scarlett Johansson as Natasha Romanoff, brought in $26.3m (£18m) in its second weekend. This marked a steep 67% decline from its $80m (£58m) inaugural sales that made it the highest-grossing post-Covid cinema release.
Also made available near-simultaneously on the Disney Plus streaming service as part of the company's multi-platform release strategy to mitigate the pandemic, it collected an additional $60m (£43m) worldwide from subscribers.