In April, Parton announced she was giving the money to Vanderbilt University Medical Center in Nashville.
That was one of the trial sites for the Moderna vaccine, which is nearly 95% effective, according to early data.
A Vanderbilt spokesperson said Parton's "generous" gift was helping "several promising research initiatives".
A portion of the singer's money went towards funding an early stage trial of the Moderna vaccine.
'Very promising research'