Kolisi leads a side which includes 11 of the players who started the World Cup final win over England in 2019.
Handre Pollard will win his 50th international cap after being selected at fly-half.
"We know what the players selected can do," said head coach Jacques Nienaber.
"The fact that most of the players participated in the Rugby World Cup means they know one another well on and off the field."
Kolisi, 30, played in the win over Georgia on 2 July, but only joined up with the squad in Cape Town on Monday after having to self-isolate for 10 days.