On Saturday, the company welcomed the first visitors to the new 34bn yuan ($5.1bn; £3.9bn) park in Hefei which also has hotels and a shopping mall.
The firm plans to build as many as 20 more such parks across China by 2020.
Dalian Wanda has been outspoken about its rivalry with US firm Walt Disney which opened its first theme park in mainland China in June.
In May, Wanda launched its first theme park in Nanchang just weeks before Disney launched its Shanghai park.
