The total number of confirmed cases now stands at 2,491, including 31 deaths.
Authorities said a lack of compliance with the "New Normal" or "Niupela Pasin" order had been a major challenge as the country continued to wrestle with the coronavirus.
The new cases come from 10 provinces, of which the National Capital District reported the 53 cases.
Additionally, there were two exported cases from PNG to a quarantine hotel in Brisbane, Australia.
Since early this year, the number of Covid-19 cases in PNG have continued to rise.